Gene Targeting and Transgenic Animal Core

The Gene Targeting and Transgenic Facility has the resources and skills necessary to construct mice with defined mutations at specific locations of the mouse genome by in vitro manipulation of embryonic stem (ES) cells by blastocyst injection of altered ES cells. In addition, transgenic mice can be prepared by DNA injection into early mouse embryos. A rederivation service is available which eliminates horizontally-transmitted pathogens from existing strains of mice and allows them to be imported into a “clean” facility. Rederivations are done by transfer of pre-implantation embryos to foster mothers. Mice with targeted mutations are prepared in the 129SvJae background but can be backcrossed to any strain. Most transgenic animals have been constructed on an F2 genetic background that derives from the parental strains of C3Hf/HeRos and C57BL/10Rospd. When necessary, however, a number of lines have been produced on other inbred strain backgrounds. For gene targeting and transgenic work, consult with the facility director prior to preparing the plasmids for use in the work to outline a strategy.

URL: http://www.roswellpark.org/document_1065_409.html

Location:
Medical Research Complex, RPCI
Roswell Park Cancer Institute

Co-directors:
Kenneth W. Gross, PhD
T: [716] 845-4572
F: [716] 845-8169
E: kenneth.gross@roswellpark.org

Steven C. Pruitt, PhD
T: [716]845-3589
F: [716] 845-8169
E: steven.pruitt@roswellpark.org

Core Manager:
Diane Poslinski
T: [716]845-1403 or 1404
F: [716] 845-5908
E: diane.poslinski@roswellpark.org