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Maldi-Tof/Proteomics
Core
The Maldi-Tof facility housed in the
Department of Biochemistry provides access to mass spectrometric
fingerprinting of unknown proteins. Maldi-Tof (Matrix-assisted,
Laser-Desorption-Ionization / Time of flight) mass spectrometry
is presently the method of choice for identification of unknown
proteins via mass analysis of proteolytic peptides, and for
characterization of post-translational modifications. This
technique is rapid, highly sensitive, and applicable to a
wide variety of research problems. Applications include direct
characterization of mutated proteins, estimating the extent
of protein derivatization (e.g., biotinylation), and identification
of unknown proteins isolated from polyacrylamide gels. Depending
on the specific application and complexity of the system,
reliable data can be obtained in the fmol-pmol range.
Location:
118
Farber Hall
UB South Campus
Director:
Kenneth Blumenthal, PhD
Tel: 716-829-2727
Fax: 716-829-2725
Email: kblumen@buffalo.edu
Manager:
Maciej Lis, PhD
Tel: 716-829-2159
Fax: 716-829-2727
Email: lis@buffalo.edu
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