Debra C. Dubois

Molecular Dynamics of Steroid Action

Research Associate Professor

B.S. (1977) McGill University
M.A. (1979), PhD. (1981) University at Buffalo
Postdoctoral work (1981) University of Maryland
Research Assistant Professor (1984);
Adjunct Assistant Professor Pharmaceutics (1995) University at Buffalo

Research Associate Professor (2001) University at Buffalo


Address Information

Debra C. DuBois
Department of Biological Sciences
117 Hochstetter Hall
State University of New York at Buffalo
Buffalo, NY 14260

(716) 645-4907

To send e-mail: dubois@buffalo.edu


RESEARCH SUMMARY

Glucocorticoids are a class of steroid hormone which regulate systemic gluconeogenesis. In addition, glucocorticoids are potent suppressors of immune system function. Synthetic glucocorticoids are widely used clinically as immunosuppressant and anti-inflammatory drugs. However, many of the side-effects of these agents result from their gluconeogenic effects on muscle, liver, kidney, and adipose tissue. A primary mechanism of action of glucocorticoids involves modulation of gene expression. The goal of our work is to apply a pharmacodynamic approach to glucocorticoid modulation of gene expression at the transcriptional and translational levels. A primary focus concerns the role of receptor autoregulation in steroid response to various steroid dosing regimens.


SELECTED PROJECTS

  • Corticosteroid Pharmacokinetics and Pharmacodynamics
  • Glucocorticoid Structure and Transcriptional Dynamics

    PUBLICATIONS

  • Ramakrishnan, R., DuBois, D., Almon, R., Pyszczynski, N., and Jusko, W.,
    Pharmacodynamics and Pharmacogenomics of Methylprednisolone During Seven-day Infusion in Rats.
    J. Pharmacology and Experimental Therapeutics, 300: 245-256, 2002
  • Ramakrishnan, R., DuBois, D., Almon, R., Pyszczynski, N., and Jusko, W.,
    Fifth- Generation Model for Corticosteroid Pharmacodynamics: Application to Steady-state Receptor Down-regulation and Enzyme Induction Patterns during Seven-day Continuous Infusion of Methylprednisolone in Rat.
    In Press J. Pharmacokinetics and Pharmacodynamics
  • Richard R. almon, Debra C. DuBois, Emily Bachman, Wei Shi, Shuzhong Zhang, Robert M. Straubinger, and William J Jusko.
    Pharmacodynamics and Pharmacogenomics of Diverse Receptor-Mediated Effects of Methylprednisolone in Rats using Microarray Analysis.
    In Press
    J. Pharmacokinetics and Pharmacodynamics
  • Almon, R.R. and DuBois, D.C.
    Tyrosine Transaminase. Encyclopedia of Molecular Biology Vol. 5: 3297-3300
  • Almon, R.R. and DuBois, D. C.
    Quantitation of expressed message for Inducible nitric oxide synthase (EC 1.14.13.39).
    In Press
    Methods in Enzymology
  • Sun, Y-N, McKAY, L.I., DuBois, D. C., Jusko, W.J., and Almon, R. R. (1999)
    Glucocorticoid Dynamics: A Pharmacokinetic/Pharmacodynamic (PK/PD) Model for Glucocorticoid Receptor Down Regulation
    and Glutamine Synthetase Induction in Rat Skeletal Muscle by a Receptor Gene Mediated Mechanism.

    J. Pharmacology and Experimental Therapeutics. 288:720-728
  • Sun, Y-N, DuBois, D. C., Almon, R. R., and Jusko, W.J (1998)
    Fourth-Generation Model for Corticosteroid Pharmacodynamics: A Model for Methylprednisolone Effects on Receptor
    Gene-Mediated Glucocorticoid Receptor Down-Regulation and Tyrosine Aminotransferase Induction in Rat Liver.

    J. Pharmacokinetics and Biopharmaceutics 26:289-317
  • DuBois, D.C., Incardona, L., Almon, R.R., Pyszczynski, N., and Jusko, W.J. (1995)
    Differential dynamics of receptor downregulation and tyrosine aminotransferase induction following glucocorticoid treatment
    J. Steroid Biochem. Mol. Biol. 54: 237
  • Xu, Z.-X., DuBois, D.C., Almon, R.R., Pyszczynski, N., and Jusko, W.J. (1995)
    Third-generation model for corticosteroid pharmacodynamics: role of MRNA for tyrosine aminotransferase enzyme in rat liver
    J. Pharmacokinetics & Biopharmaceutics 23: 163
  • DuBois, D.C., Almon, R.R., and Jusko, W.J. (1993)
    Molar quantification of specific messenger ribonucleic acid expression in Northern hybridization using CRNA standards
    Anal. Biochem. 210: 140

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