Debra C. Dubois
Molecular Dynamics of Steroid Action
Research Associate Professor
B.S. (1977) McGill University
M.A. (1979), PhD. (1981) University at Buffalo
Postdoctoral work (1981) University of Maryland
Research Assistant Professor (1984);
Adjunct Assistant Professor Pharmaceutics (1995) University at
Buffalo
Research Associate Professor (2001) University at Buffalo
Address Information
Debra C. DuBois
Department of Biological Sciences
117 Hochstetter Hall
State University of New York at Buffalo
Buffalo, NY 14260
(716) 645-2363 ext: 114
To send e-mail: dubois@nsm.buffalo.edu
RESEARCH SUMMARY
Glucocorticoids are a class of steroid
hormone which regulate systemic gluconeogenesis. In addition,
glucocorticoids are potent suppressors of immune system function.
Synthetic glucocorticoids are widely used clinically as
immunosuppressant and anti-inflammatory drugs. However, many of
the side-effects of these agents result from their gluconeogenic
effects on muscle, liver, kidney, and adipose tissue. A primary
mechanism of action of glucocorticoids involves modulation of
gene expression. The goal of our work is to apply a
pharmacodynamic approach to glucocorticoid modulation of gene
expression at the transcriptional and translational levels. A
primary focus concerns the role of receptor autoregulation in
steroid response to various steroid dosing regimens.
SELECTED PROJECTS
Corticosteroid Pharmacokinetics and
Pharmacodynamics
Glucocorticoid Structure and Transcriptional
Dynamics
PUBLICATIONS
Ramakrishnan, R., DuBois, D., Almon, R., Pyszczynski, N.,
and Jusko, W.,
Pharmacodynamics and Pharmacogenomics of
Methylprednisolone During Seven-day Infusion in Rats.
J. Pharmacology and Experimental Therapeutics, 300:
245-256, 2002
Ramakrishnan, R., DuBois, D., Almon, R., Pyszczynski, N.,
and Jusko, W.,
Fifth- Generation Model for Corticosteroid
Pharmacodynamics: Application to Steady-state Receptor
Down-regulation and Enzyme Induction Patterns during
Seven-day Continuous Infusion of Methylprednisolone in
Rat.
In Press J. Pharmacokinetics and
Pharmacodynamics
Richard R. almon, Debra C. DuBois, Emily Bachman, Wei
Shi, Shuzhong Zhang, Robert M. Straubinger, and William J
Jusko.
Pharmacodynamics and Pharmacogenomics of Diverse
Receptor-Mediated Effects of Methylprednisolone in Rats
using Microarray Analysis.
In Press J. Pharmacokinetics and
Pharmacodynamics
Almon, R.R. and DuBois, D.C.
Tyrosine Transaminase. Encyclopedia of
Molecular Biology Vol. 5: 3297-3300
Almon, R.R. and DuBois, D. C.
Quantitation of expressed message for Inducible
nitric oxide synthase (EC 1.14.13.39).
In Press Methods in Enzymology
Sun, Y-N, McKAY, L.I., DuBois, D. C., Jusko, W.J., and
Almon, R. R. (1999)
Glucocorticoid Dynamics: A
Pharmacokinetic/Pharmacodynamic (PK/PD) Model for
Glucocorticoid Receptor Down Regulation
and Glutamine Synthetase Induction in Rat Skeletal Muscle
by a Receptor Gene Mediated Mechanism.
J. Pharmacology and Experimental Therapeutics.
288:720-728
Sun, Y-N, DuBois, D. C., Almon, R. R., and Jusko, W.J
(1998)
Fourth-Generation Model for Corticosteroid
Pharmacodynamics: A Model for Methylprednisolone Effects
on Receptor
Gene-Mediated Glucocorticoid Receptor Down-Regulation and
Tyrosine Aminotransferase Induction in Rat Liver.
J. Pharmacokinetics and Biopharmaceutics
26:289-317
DuBois, D.C., Incardona, L., Almon, R.R., Pyszczynski,
N., and Jusko, W.J. (1995)
Differential dynamics of receptor downregulation and
tyrosine aminotransferase induction following
glucocorticoid treatment
J. Steroid Biochem. Mol. Biol. 54: 237
Xu, Z.-X., DuBois, D.C., Almon, R.R., Pyszczynski, N.,
and Jusko, W.J. (1995)
Third-generation model for corticosteroid
pharmacodynamics: role of MRNA for tyrosine
aminotransferase enzyme in rat liver
J. Pharmacokinetics & Biopharmaceutics
23: 163
DuBois, D.C., Almon, R.R., and Jusko, W.J. (1993)
Molar quantification of specific messenger
ribonucleic acid expression in Northern hybridization
using CRNA standards
Anal. Biochem. 210: 140
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